Dionex Ultimate 3000 Hplc User Manual

UltiMate 3000 Basic Manual LC System

UltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page 1 1 Introduction 1.1 How to Use this Manual The layout of this manual is designed to provide quick reference to the sections of interest to the reader when operating the Thermo Scientific™ Dionex™ UltiMate™ 3000 pump.

Dionex Ultimate 3000 Hplc User Manual Download

The UltiMate® 3000 Basic Manual System includes a manual injector and is suitable for classical isocratic or quaternary gradient LC separations at ambient temperature and analytical flow rates.

Dionex
  • Cost-effective access to UHPLC separations at up to 62 MPa (9000 psi)
  • Excellent retention time and gradient precision due to SmartFlow® technology
  • Manual injection (full- and partial-loop)
  • High detection sensitivity with low detector noise and drift
  • Robust, dependable performance at a low cost of ownership

The UltiMate 3000 Basic Manual System typically includes the ISO-3100SD Isocratic Analytical Pump or LPG-3400SD Quaternary Analytical Pump. High detection sensitivity is guaranteed by the VWD-3100 Variable Wavelength Detector with data rates of up to 100 Hz.

PUMPS
Recommended PumpLPG-3400SD
Flow Range
(Settable Range)
0.2–10 mL/min
(0.001–10 mL/min)
Flow Accuracy±0.1%
Flow Precision<0.05% RSD or <0.01 min SD, whichever is greater
Pressure Range2–62 MPa (290–9000 psi)
No. of Eluent Lines4
Gradient Delay Volume690 µL by default (325–1790 µL with optional mixer kits), independent of operating pressure
Biocompatible VersionLPG-3400AB (titanium)
Solvent DegassingBuilt-in, 4 channels
DETECTOR
Recommended DetectorsVWD-3100
Detector TypeVariable Wavelength
Number of Channels1
Wavelength Range190–900 nm
Max. Data Collection Rate100 Hz
Recommened Flow CellAnalytical: 11 µL, 10 mm path
Noise<±3.5 µAU at 254 nm
Drift<0.1 µAU/h
3000

Performance Examples

UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.

User

Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.

UltiMate 3000 RSLC Systems
Conventional and UHPLC Separations
Robust Viper Connections
Outstanding Precision with the UltiMate 3000 RSLC System
Efficiently Accelerate Existing HPLC Methods with RSLC
10 Peaks in 10 Seconds
Almost Zero Autosampler Carry-Over
Short UltiMate 3000 Cycle Times for Maximum Throughput
Constant and Independent Column Temperature Control
Efficient Precolumn Heating
Precise Peak Identification with RSLC Methods
Faster Results in Chemical Process Control
Instant Process Control Results
Fast Separation of Eight Softdrink Additives
Ultra-High Resolution Analysis of 29 Pesticides
Reduction of Eluent Consumption
Speed-up of Complex Separations
Ultrafast Amino Acid Analysis
Fast Amino Acid Analysis on a Sub-2 µm Column
Performance
Enhanced Isocratic Retention Time Precision
Enhanced Gradient Retention Time Precision
Reduced Total Cycle Time
Enhanced Autosampler Injection Volume Precision
Enhanced Impurity Quantification
Reduced Detector Noise and Drift
Enhanced Detector Linearity
Enhanced Autosampler Linearity
Enhanced Autosampler Temperature Accuracy
Enhanced Autosampler Injection Volume Accuracy
Reliability
Enhanced System to System Reproducibility
Enhanced Column Compartment Temperature Stability
Product Brochures
UltiMate 3000 Basic Automated System
UltiMate 3000 Liquid Chromatography Systems Brochure
Product Data Sheets
ACC-3000 Autosampler Column Compartment

The following LC modules and accessories represent a typical UltiMate 3000 Basic Manual system configuration. Due to its modular setup, the system can be upgraded easily by adding or exchanging LC modules.

Hardware
SR-3000 UltiMate 3000 Solvent Rack without Degasser5035.9200
ISO-3100SD - UltiMate 3000 Isocratic Analytical Pump5040.0011
LPG-3400SD - UltiMate 3000 Quaternary Analytical Pump5040.0031
VWD-3100 - UltiMate 3000 Single Channel Variable Wavelength Detector (Without Flow Cell)5074.0005

Dionex Ultimate 3000 Hplc User Manual Instructions

Accessories
100 µL Syringe for Manual Injectors6035.0665
Analytical Flow Cell for VWD-3000 Series, SST, 11 µL Volume, 10 mm Pathlength6074.0250
Analytical Manual Injection Valve Kit for UltiMate 3000 2G Pump Series incl. 20 µL Sample Loop and Pump Mounting Bracket 6040.0610

Dionex Ultimate 3000 Hplc

  • Chromatography Data Systems

Dionex Ultimate 3000 Autosampler Manual

  • LC Modules