UltiMate 3000 Basic Manual LC System
UltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page 1 1 Introduction 1.1 How to Use this Manual The layout of this manual is designed to provide quick reference to the sections of interest to the reader when operating the Thermo Scientific™ Dionex™ UltiMate™ 3000 pump.
Dionex Ultimate 3000 Hplc User Manual Download
The UltiMate® 3000 Basic Manual System includes a manual injector and is suitable for classical isocratic or quaternary gradient LC separations at ambient temperature and analytical flow rates.
- Cost-effective access to UHPLC separations at up to 62 MPa (9000 psi)
- Excellent retention time and gradient precision due to SmartFlow® technology
- Manual injection (full- and partial-loop)
- High detection sensitivity with low detector noise and drift
- Robust, dependable performance at a low cost of ownership
The UltiMate 3000 Basic Manual System typically includes the ISO-3100SD Isocratic Analytical Pump or LPG-3400SD Quaternary Analytical Pump. High detection sensitivity is guaranteed by the VWD-3100 Variable Wavelength Detector with data rates of up to 100 Hz.
| PUMPS |
|---|
| Recommended Pump | LPG-3400SD |
Flow Range
(Settable Range) | 0.2–10 mL/min
(0.001–10 mL/min) |
| Flow Accuracy | ±0.1% |
| Flow Precision | <0.05% RSD or <0.01 min SD, whichever is greater |
| Pressure Range | 2–62 MPa (290–9000 psi) |
| No. of Eluent Lines | 4 |
| Gradient Delay Volume | 690 µL by default (325–1790 µL with optional mixer kits), independent of operating pressure |
| Biocompatible Version | LPG-3400AB (titanium) |
| Solvent Degassing | Built-in, 4 channels |
| DETECTOR |
|---|
| Recommended Detectors | VWD-3100 |
| Detector Type | Variable Wavelength |
| Number of Channels | 1 |
| Wavelength Range | 190–900 nm |
| Max. Data Collection Rate | 100 Hz |
| Recommened Flow Cell | Analytical: 11 µL, 10 mm path |
| Noise | <±3.5 µAU at 254 nm |
| Drift | <0.1 µAU/h |
Performance Examples
UltiMate® 3000 HPLC systems deliver improvements in HPLC performance while ensuring easy, reliable operation.The UltiMate 3000 series operates over a wide range of flow rates, including preparative, analytical, micro, and nano/capillary scales, supporting applications such as discovery processes, quality control, and advanced research like proteomics.
Rapid Separation LC offers the highest flow-pressure range to meet your chromatographic goals whatever the may be. Developing separations in seconds rather than in minutes requires a system that provides excellent precision, speed, and performance. Use the RSLC Method Transfer Calculator for quick and easy conversion of conventional HPLC to UHPLC methods.
| UltiMate 3000 RSLC Systems |
|---|
| Conventional and UHPLC Separations |
| Robust Viper Connections |
| Outstanding Precision with the UltiMate 3000 RSLC System |
| Efficiently Accelerate Existing HPLC Methods with RSLC |
| 10 Peaks in 10 Seconds |
| Almost Zero Autosampler Carry-Over |
| Short UltiMate 3000 Cycle Times for Maximum Throughput |
| Constant and Independent Column Temperature Control |
| Efficient Precolumn Heating |
| Precise Peak Identification with RSLC Methods |
| Faster Results in Chemical Process Control |
| Instant Process Control Results |
| Fast Separation of Eight Softdrink Additives |
| Ultra-High Resolution Analysis of 29 Pesticides |
| Reduction of Eluent Consumption |
| Speed-up of Complex Separations |
| Ultrafast Amino Acid Analysis |
| Fast Amino Acid Analysis on a Sub-2 µm Column |
| Performance |
|---|
| Enhanced Isocratic Retention Time Precision |
| Enhanced Gradient Retention Time Precision |
| Reduced Total Cycle Time |
| Enhanced Autosampler Injection Volume Precision |
| Enhanced Impurity Quantification |
| Reduced Detector Noise and Drift |
| Enhanced Detector Linearity |
| Enhanced Autosampler Linearity |
| Enhanced Autosampler Temperature Accuracy |
| Enhanced Autosampler Injection Volume Accuracy |
| Reliability |
|---|
| Enhanced System to System Reproducibility |
| Enhanced Column Compartment Temperature Stability |
| Product Brochures |
|---|
| UltiMate 3000 Basic Automated System |
| UltiMate 3000 Liquid Chromatography Systems Brochure |
| Product Data Sheets |
|---|
| ACC-3000 Autosampler Column Compartment |
The following LC modules and accessories represent a typical UltiMate 3000 Basic Manual system configuration. Due to its modular setup, the system can be upgraded easily by adding or exchanging LC modules.
| Hardware |
|---|
| SR-3000 UltiMate 3000 Solvent Rack without Degasser | 5035.9200 |
| ISO-3100SD - UltiMate 3000 Isocratic Analytical Pump | 5040.0011 |
| LPG-3400SD - UltiMate 3000 Quaternary Analytical Pump | 5040.0031 |
| VWD-3100 - UltiMate 3000 Single Channel Variable Wavelength Detector (Without Flow Cell) | 5074.0005 |
Dionex Ultimate 3000 Hplc User Manual Instructions
| Accessories |
|---|
| 100 µL Syringe for Manual Injectors | 6035.0665 |
| Analytical Flow Cell for VWD-3000 Series, SST, 11 µL Volume, 10 mm Pathlength | 6074.0250 |
| Analytical Manual Injection Valve Kit for UltiMate 3000 2G Pump Series incl. 20 µL Sample Loop and Pump Mounting Bracket | 6040.0610 |
Dionex Ultimate 3000 Hplc
- Chromatography Data Systems
Dionex Ultimate 3000 Autosampler Manual